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palmitic acid  (Revvity)


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    Structured Review

    Revvity palmitic acid
    Palmitic Acid, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3h+palmitic+acid/Palmitic+Acid%2C+%5B9%2C10-3H(N)%5D-%2C+1mCi/pmc10419124-259-12-15
    Average 91 stars, based on 118 article reviews
    palmitic acid - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Labeling:

    Article Title: Site-specific S-Acylation of Influenza Virus Hemagglutinin
    Article Snippet: .. Cells were labeled at 5.5 h postinfection with 0.5 mCi/ml [35S]methionine/cysteine (1,175 Ci/mmol; Easy Tag Express Protein Labeling Mix, PerkinElmer Life Sciences) or with 0.25 mCi/ml [3H]palmitic acid (30 – 60 Ci/mmol; 9,10-3H; PerkinElmer Life Sciences) for 3 h. Cells were lysed in radioimmune precipitation assay buffer (20 mM Tris-HCl, 150 mM NaCl, 10 mM EDTA, 10 mM iodoacetamide, 0.1% (w/v) SDS, 1% (v/v) Triton X-100, and 1% (w/v) sodium deoxycholate, pH 7.4), cell debris was pelleted, and the cleared supernatant was incubated at 4 °C for 16 h with 1 l of anti-influenza H1N1 virus polyclonal rabbit antibody R309 (32) kindly provided by Y. Kawaoka. ..

    Article Title: Palmitoylation of Amyloid Precursor Protein Regulates Amyloidogenic Processing in Lipid Rafts
    Article Snippet: .. CHO-APP cells were metabolically labeled with [ 3H] palmitic acid ([ 3H]-C16; PerkinElmer) as described previously (Vetrivel et al., 2009). ..

    Article Title: The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts
    Article Snippet: Transfected HEK293T cells were preincubated for 30 min in serum-free DMEM with fatty acid-free bovine serum albumin (5 mg/ml; Sigma-Aldrich). .. Cells were metabolically labeled with 0.4 mCi/ml [3H]palmitic acid (PerkinElmer) for 4 h in the preincubation buffer. ..

    Incubation:

    Article Title: Site-specific S-Acylation of Influenza Virus Hemagglutinin
    Article Snippet: .. Cells were labeled at 5.5 h postinfection with 0.5 mCi/ml [35S]methionine/cysteine (1,175 Ci/mmol; Easy Tag Express Protein Labeling Mix, PerkinElmer Life Sciences) or with 0.25 mCi/ml [3H]palmitic acid (30 – 60 Ci/mmol; 9,10-3H; PerkinElmer Life Sciences) for 3 h. Cells were lysed in radioimmune precipitation assay buffer (20 mM Tris-HCl, 150 mM NaCl, 10 mM EDTA, 10 mM iodoacetamide, 0.1% (w/v) SDS, 1% (v/v) Triton X-100, and 1% (w/v) sodium deoxycholate, pH 7.4), cell debris was pelleted, and the cleared supernatant was incubated at 4 °C for 16 h with 1 l of anti-influenza H1N1 virus polyclonal rabbit antibody R309 (32) kindly provided by Y. Kawaoka. ..

    Virus:

    Article Title: Site-specific S-Acylation of Influenza Virus Hemagglutinin
    Article Snippet: .. Cells were labeled at 5.5 h postinfection with 0.5 mCi/ml [35S]methionine/cysteine (1,175 Ci/mmol; Easy Tag Express Protein Labeling Mix, PerkinElmer Life Sciences) or with 0.25 mCi/ml [3H]palmitic acid (30 – 60 Ci/mmol; 9,10-3H; PerkinElmer Life Sciences) for 3 h. Cells were lysed in radioimmune precipitation assay buffer (20 mM Tris-HCl, 150 mM NaCl, 10 mM EDTA, 10 mM iodoacetamide, 0.1% (w/v) SDS, 1% (v/v) Triton X-100, and 1% (w/v) sodium deoxycholate, pH 7.4), cell debris was pelleted, and the cleared supernatant was incubated at 4 °C for 16 h with 1 l of anti-influenza H1N1 virus polyclonal rabbit antibody R309 (32) kindly provided by Y. Kawaoka. ..

    Lysis:

    Article Title: S-palmitoylation regulates biogenesis of core glycosylated wild-type and F508del CFTR in a post-ER compartment
    Article Snippet: .. Following serum deprivation, cells were labelled with 500 μCi/ml [3H]palmitic acid (PerkinElmer) for 4 h in DMEM as above, rinsed and collected in ice-cold PBS with lysis in RIPA buffer [150 mM NaCl, 1% Nonidet P40, 0.5% deoxycholate, 0.1% deoxycholate, 0.1% SDS and 50 mM Tris/HCl (pH 7.4)]. .. CFTR was immunoprecipitated using 24-1 or 10B6.2 antibody conjugated to Protein A/G–agarose (Thermo Scientific Pierce Protein Biology Products), followed with rinsing and release by 37 ◦C incubation in 4× Laemmli sample buffer containing 10 % 2-mercaptoethanol.

    other:

    Article Title: Phospholipase D-mTOR signaling is compromised in a rat model of depression.
    Article Snippet: Depression is associated with structural and neurochemical changes in limbic structures, including the hippocampus, that control emotion and mood.. Structural abnormalities such as decrease in hippocampal cell proliferation, neurogenesis and hippocampal volume, and loss of neurons and glial cells have been widely reported in physical and psychosocial stress paradigms and animal model of depression, but corresponding neurochemical changes are largely unknown.. Using neonatal clomipramine (CL)-treated rats as a model to elucidate the association of phospholipase D (PLD) and mammalian target of rapamycin (mTOR) signaling with depressive pathology, we found that the hippocampus of CL-treated rats showed significantly down-regulation of PLD1 expression and attenuation of PLD activity which leads to the less formation of phosphatidic acid (PA), an activator of mTOR, and free choline, a potential biomarker for depression.

    Metabolic Labelling:

    Article Title: Palmitoylation of Amyloid Precursor Protein Regulates Amyloidogenic Processing in Lipid Rafts
    Article Snippet: .. CHO-APP cells were metabolically labeled with [ 3H] palmitic acid ([ 3H]-C16; PerkinElmer) as described previously (Vetrivel et al., 2009). ..

    Article Title: The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts
    Article Snippet: Transfected HEK293T cells were preincubated for 30 min in serum-free DMEM with fatty acid-free bovine serum albumin (5 mg/ml; Sigma-Aldrich). .. Cells were metabolically labeled with 0.4 mCi/ml [3H]palmitic acid (PerkinElmer) for 4 h in the preincubation buffer. ..



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    Image Search Results


    Journal: Cell reports

    Article Title: Palmitoylation and PDE6δ regulate membrane-compartment-specific substrate ubiquitylation and degradation

    doi: 10.1016/j.celrep.2023.111999

    Figure Lengend Snippet:

    Article Snippet: [ 3 H] palmitic acid , PerkinElmer , Cat# NET043001MC.

    Techniques: Recombinant, Transfection, Mass Spectrometry, Software, Knock-In, Clone Assay, Expressing, Plasmid Preparation